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when it comes to detection you know of course I have to put the pitch in here that says you want to buy an agra serie antibody of course but the key is to match your antibody concentration to your detection system and to the load of anti protein that you put on the blot in the first place you have to have more primary antibody there than you have target molecules on the block because it wonamp;#39;t be quantitative unless youamp;#39;re in an excess antibody situation and that doesnamp;#39;t really matter if youamp;#39;re doing pretty pictures or antibodies but if youamp;#39;re doing quantitative immunoblotting then you really have to have an excess of a primary antibody it depends a lot on what detection system youamp;#39;re using my lab uses ECL advanced which is from GE and my normal pretty picture antibody constant dilution would be one in fifty thousand I go to one in twenty five thousand if Iamp;#39;m doing quantitative because I want to make sure that I have more molecules
