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chemiluminescent detection has been the method of choice for Western blot analysis because of its high sensitivity high signal-to-noise ratios and versatility for use with film or digital imagers using this approach a membrane is probed with an antibody that is conjugated to an enzyme usually HRP and a chemiluminescent substrate the majority of chemiluminescent HRP substrates are two component systems consisting of a stable peroxide solution and an enhanced luminol or acridine solution when incubated with a block to which h RP conjugated antibodies or other probes are bound a chemical reaction emits light at 425 nanometers this light can be captured with x-ray film CCD camera imaging devices or phosphor integers that detect chemiluminescence thermo scientific super signal chemiluminescent h RP substrates provide a range of convenient and reliable solutions for Western blot analysis that vary in terms of detection sensitivity and signal duration in while commercial ECL substrate