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this is USMLE QA USMLE material presented in a question and answer format structured similar to the first date for the USMLE step one book question what is a western blot answer a western blood is a technique used to detect specific proteins in a sample by separating the proteins via gel electrophoresis and transferring them to a membrane question how is the sample protein separated in a western blood answer sample protein is separated via gel electrophoresis than a western blot question what is used to bind to the relevant protein in a western blood answer a labeled antibody is used to bind to the relevant protein in a western blood question what happens after the labeled antibody binds to the relevant protein in a western blood answer the next step is typically to detect The Binding of the labeled antibody to the protein which can be done using a chemoluminescent or fluorescent substrate question what is the purpose of a western blood answer the purpose of a western blood is to detec
