Blot answer in SE

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How to blot answer in SE

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hello mark hello everybody out there welcome to the todayamp;#39;s webinar about Western plotting and the troubles you can have with this method first of all I want to give you an overview about the Western plot feature and then I will show you possible problems can be revealed by the detection system so what can go wrong on how can you solve the problems and at the end you will see the way how to get a good Western plot so letamp;#39;s start with the Western plotting workflow so you can see itamp;#39;s a quite complex method with different steps so why do you perform a Western plotting analyzes so first of all you want to confirm the presence of a target protein in a special cell acid or tissue preparation and you want to obtain FEMA quantitative information so if a certain target protein is regulated in a special way by a special substances and these things so hmm since we have technical problems okay here we are first of all thatamp;#39;s the sample preparations and this is at t

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What diseases are diagnosed by western blotting?

Western blotting is frequently used for the confirmatory medical diagnosis of infectious diseases such as Lyme disease, HIV infection, bovine spongiform encephalopathy (BSE), hepatitis C infection, syphilis, inflammatory muscle conditions such as myositis, and certain autoimmune disorders (e.g., paraneoplastic disease)

What is blot test for?

The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection.

What does a positive Western blot test mean?

Only a positive Western blot test can confirm the diagnosis of Lyme disease. For many people, the ELISA test remains positive, even after they have been treated for Lyme disease and no longer have symptoms.

What are the results of a Southern blot?

The results of a Southern blot are observed in the form of bands on the membrane. The size of the DNA fragments can be determined by comparing their relative size with the DNA bands of known lengths.

What is the purpose of a blot?

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells.

How do you detect Southern Blot?

Detection: After hybridization, excess probe is washed from the membrane (typically using SSC buffer), and the pattern of hybridization is visualized on X-ray film by autoradiography in the case of a radioactive or fluorescent probe, or by development of color on the membrane if a chromogenic detection method is used.

What does blot mean in science?

In molecular biology and genetics, a blot is a method of transferring large biomolecules (proteins, DNA or RNA) onto a carrier, such as a membrane composed of nitrocellulose, polyvinylidene fluoride or nylon.

What is Western blot test done to confirm?

The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test sometimes produces false positives, a second test is needed to further the diagnosis.

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