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With chemiluminescent Western blot detection, the signal-producing chemicals can be washed away and the antibodies stripped from the membrane so that the blot can be re-probed for another target protein. This is possible because the chemiluminescent light emitted by the HRP-oxidized substrate is not bound to the blot like with colored precipitates. Various stripping buffer formulations are commercially available or can be made in the lab. These buffers generally include a detergent and reducing agent, Some also require heat and/or low pH to effectively strip off antibodies. To strip a blot of all antibodies and chemiluminescent reagents, immerse the blot in stripping buffer and either incubate with agitation or heat in an oven, depending on the protocol used. After a specific incubation period, wash the blot thoroughly to ensure that all stripping reagents are removed. When using stripping buffers with beta-mercaptoethanol as the reducing agent, you will know that your blot has been su