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in this Final Phase we will demonstrate signal development using the most common most sensitive and most inexpensive detection method the electroch chemoluminescent or ecl reaction this method utilizes the hrp enzyme which was conjugated to the secondary to catalyze the ecl reaction and produce light the light is then gathered onto x-ray film and developed or digitized with the aid of a specialized camera sens it enough for this application we start by mixing equal parts ecl reagents in a 1:1 ratio ing to the manufactureramp;#39;s instructions we will incubate the membrane for 3 to 5 minutes without agitation after incubation decant ecl mixture and use a laboratory wipe to Wick off excess solution from the corner of the membrane place the membrane in a clear plastic wrap such as a sheet protector to prevent drying avoid letting the membrane completely dry out we can now use a roller to push out any bubbles or excess solution immediately develop the membrane both film and camera system
