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Andy Alhassan: Onchocerciasis, or river blindness as it is commonly known, is one of the neglected tropical diseases with great impact on health for people in Africa, some parts of Latin America and the Yemen peninsula. There is the need for a proper, specific, and sensitive differential diagnosis for this disease. Andy Alhassan: The parasite is a worm called Onchocerca volvulus. People get infection through the bites of these black flies. So, to determine the level of infection these flies are caught, dissected, to detect these parasites. However, this methodology is quite tedious, time-consuming, and sometimes the method is not sufficient for proper identification of these worms. Andy Alhassan: Again, we have PCR techniques, which are used to detect parasite DNA in these flies. The cattle parasite and the human parasite are so closely related that the PCR biomarker used cannot differentiate between the two. Hence, there been a need for a proper method that can be used to differentiat