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okay now letamp;#39;s look at an actual binding curve that weamp;#39;ve studied before it may it may apply to an eliza interacting with letamp;#39;s say a protein on the surface of a plate with antibodies in a subsequent detection antibody may be a little bit more complicated this is our simple equilibrium macromolecule plus ligand forming a complex and we know that if we plot fractional saturation which can go from zero to one versus the free ligand concentration we get a hyperbola and this is the equation this is a basically equation for a rectangular hyperbola and if I were to ask you thereamp;#39;s only will only one parameter in this and thatamp;#39;s KD that would be enough to fit this data but you could not fit it with a linear fit this has to be fit to a nonlinear equation now thereamp;#39;s another way to plot this data and that would be to plot instead the log about instead of how this is often done and you can see that this sort of makes this curve rise a little bit mo
