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The Aggregate Variants per Gene tool aggregates the VCF files in the specified folder for each gene and mutation type. After dividing the sample into the Control Group and Case Group, press the Aggregate button to run the aggregation. Check only the missense and frameshift variants for the variant type and press the Filter button. In the Population Filter, we try to extract the mutations that do not exist in Control Group but always appear in Case Group. There are 238 genes found, letamp;#39;s see them by pressing the Export Table button. The results are stored in amp;quot;aggregate per geneamp;quot; folder; the TSV file is a table of aggregate products. There are columns for gene names, number of occurrences of each sample, mean, frequency in the Control Group. Those in the Case Group follow. This is a list of genes having mutations in the Case Group. And the number of frameshift and missense variants for each gene. Return to the tools screen and press the Export BED button. You fi