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so welcome to this troubleshooting video on elastin stains weamp;#39;re going to start with some very similar sections to the ones that were stained in the protocol video these first ones are the Hoffs van Giessen applied to the sections of esophagus starting with his first slide here this is actually one of the slides that we stained during the protocol video and probably not perfect but itamp;#39;s itamp;#39;s pretty good in terms of first of all the the amount of elastin staining here is very clear as shown this artery the internal elastic lamina is well contrasted looking at the collagen itamp;#39;s looking pretty bright pink on the monitor and perhaps less so down the eyepieces but itamp;#39;s not far off where youamp;#39;d want it to be so itamp;#39;s been well differentiated to a point where the background is light but you havenamp;#39;t overdone it such that the elastin still looks quite quite good if we just look at the mucin within these glandular structures h
