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hello everyone my name is Celia and Iamp;#39;m an application specialist in Byram company we continue our videos about histological status after precession in Baden and section we have to stain the tissue to observe its structure using light microscopy one of the most popular and principal stains in histology is hematoxylin and as in steam it gives us an overview of the tissue and its structure hematoxylin binds with buzzer philic components of the tissue for example DNA and RNA so we can observe nuclei stains in blue or purple color as in binds with acidophilic substances such as positively charged amino acid side chains so as a result the cytoplasm is pink or orange all the samples in laboratory are stained with this method there are several different types of hematoxylin and essence and they can give us different results the result of the staining can vary depending on manufacturers including quality of solutions type of tissue protocols of the prior steps and even quality