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the any base changer tool makes designing back-to-back primers for using the q5 site-directed mutagenesis kit a quick and easy process to begin select the new sequence button then paste your starting or while type plasmid sequence in raw or fast stuff format alternatively you can choose from one of the common vector sequences available in the drop down menu if desired you can add a sequence name to the file which will populate on the summary next set the mutagenesis type by choosing substitution insertion or deletion from the options on the right for substitutions and deletions select the nucleotides to be changed or deleted for insertions select the nucleotides that flank the desired insertion site you may click and drag within the display sequence to select a range you may also use the find sequence field to search within your plasmid for your region of interest once this is found you can select by clicking and dragging over the highlighted area when making a selection the first and