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this video presents a set of four experiments used for identifying functional hiv1nf protein motifs and for developing peptide based inhibitors specific short peptides derived from motifs found in full-length proteins both retain their biological function and competitively inhibit the function of the full-length protein to identify apoptotic motifs in hiv-1 neph jur cat cells are exposed to neph-scanning peptides and a tunnel apoptotic assay is performed to identify peptides that induce apoptosis a second set of peptides containing variations on a neph secretion motif the secretion modification region or smr are transfected into cells along with neff gfp loss of function due to competitive inhibition indicated by decreased secretion of neph gfp is assessed by fluorimetry to identify cellular factors that interact with the secretion modification region or smr of nf co-immunoprecipitations are performed using smr wild-type neff as bait finally to test whether these interactions a
