Bind motif in VIA

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Aug 6th, 2022
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  1. Add your VIA to your account by clicking the New Document and selecting how you want to add your VIA file.
  2. Open your file in our editor.
  3. Make your desired edits using drag and drop tools.
  4. Once completed, click Download/Export and save your VIA to your device or cloud storage.
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How to bind motif in VIA

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so hello everyone today we will be discussing about the about the helix-turn-helix motive among the 4 different types of DNA binding domains if you remember when we started talking about the DNA binding domains we discussed that we will look at four different types of DNA binding domains helix-turn-helix motive though we will also include homeodomain motives this is a special class of helix-turn-helix motives this doesnamp;#39;t mind a difference between the two and then I will talk about the basically helix-loop-helix motive than zinc finger motive and then leucine zipper motive I hope you remember that in one of the previous lectures we we talked about the DNA binding domains that these domains can have that these proteins can have a specific transcription factors for example they can have specific regions of the proteins which can interact or bind to the DNA they can interact with the DNA so these regions which bind to the DNA or interact with the DNA we call them the DNA binding d

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A binding motif pair consists of two motifs each derived from one side of the binding protein sequences. The discovery is a directed approach that uses a combination of two data sources: 3-D structures of protein complexes and sequences of interacting proteins.
These motifs generally use either helices or sheets to bind to the major groove of DNA; this groove, as we have seen, contains sufficient information to distinguish one DNA sequence from any other.
Transcription factor binding motifs (TFBMs) are genomic sequences that specifically bind to transcription factors. The consensus sequence of a TFBM is variable, and there are a number of possible bases at certain positions in the motif, whereas other positions have a fixed base.
In the human genome, the most common types of markers are SNPs, STRs, and indels. SNPs affect only one of the basic building blocksadenine (A), guanine (G), thymine (T), or cytosine (C)in a DNA segment.
Usually, interaction with the sugarphosphate backbone of the DNA molecules is nonsequence-specific. The helixturnhelix (HTH) motif is the most common motif found in DNA-binding proteins in both prokaryotes and eukaryotes.
Which item is a common DNA‑binding motif? induction.
Thus far, at least five types of DNA-binding motifs have been extensively characterized. These include, (1) helix-turn-helix (HTH) motif, (2) leucine zipper motif, (3) helix-loop-helix (HLH) motif, (4) zinc finger motif, and (5) steroid-hormone-binding motif.
Among the most prevalent of DNA-binding domains in prokaryotes are the helix-turn-helix and winged-helix structures that place an -helix for interaction with bases in the major groove of the target DNA sequence (Harrison and Aggarwal, 1990; Brennan, 1993).

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