Bind motif in NBP

Use this walkthrough to bind motif in NBP quickly

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How to bind motif in NBP

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This presentation shows the steps for someone who is interested in finding both the sequence level promoter motifs of yeast genes plus the transcription factors that regulate their expression. If a researcher is interested in ultimately finding networks regulation but first wants to check specific favorite genes of interest, then he can follow these steps. Start by opening the website of Yeastract-- ww.yeastract.com. A window will appear with an introduction to associated tools. Also, several options are given in blue on the left-hand side of the window. These will be used in this tutorial. When clicking on them, theyamp;#39;ll change the color into purple as a reminder to which options have been used. For example, click on Search for TFs, or transcription factors, under Regulatory Associations in the left-hand column of the home page. Now, a window will open where you can type your sequence ID or name, like, for example, cdc24. Click Search to get the transcription factor, TF. You ca

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What is the ATP binding motif?

An ATP-binding motif is a 250-residue sequence within an ATP-binding proteins primary structure. The binding motif is associated with a proteins structure and/or function. ATP is a molecule of energy, and can be a coenzyme, involved in a number of biological reactions.

What is a motif in a protein sequence?

A motif is a short conserved sequence pattern associated with distinct functions of a protein or DNA. It is often associated with a distinct structural site performing a particular function. A typical motif, such as a Zn-finger motif, is ten to twenty amino acids long.

What is the difference between free and bound motif?

The motifs which cannot be omitted are bound motifs; those which may be omitted without disturbing the whole causal-chronological course of events are free motifs (Thematics 68).

Which is the most common DNA-binding motif?

Usually, interaction with the sugarphosphate backbone of the DNA molecules is nonsequence-specific. The helixturnhelix (HTH) motif is the most common motif found in DNA-binding proteins in both prokaryotes and eukaryotes.

What is a binding motif?

Transcription factor binding motifs (TFBMs) are genomic sequences that specifically bind to transcription factors. The consensus sequence of a TFBM is variable, and there are a number of possible bases at certain positions in the motif, whereas other positions have a fixed base.

What are RNA-binding motifs?

RNA-binding motif (RBM) proteins are a group of loosely related RRM-containing RBPs (Sutherland et al., 2005). Several RBM proteins have been implicated in cardiomyocyte differentiation and myofibrillogenesis. One of the strongest candidates is RBM24.

Where do DNA-binding motifs bind to?

These motifs generally use either helices or sheets to bind to the major groove of DNA; this groove, as we have seen, contains sufficient information to distinguish one DNA sequence from any other.

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