Bind motif in NB

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How to bind motif in NB

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thank you hi everyone welcome back to my channel I am so happy to see you all here so in this video we are going to talk about some of the downstream analysis that we can perform with certain types of high throughput data and we will be talking about that in further detail but the highlight of todayamp;#39;s topic is going to be motive Discovery and Performing transcription Factor binding site analysis looking for transcription Factor binding sites looking for motives but before we go into that we are going to talk about what are motives what are transcription factors um how do transcription Factor regulate or control the expression of genes how are these motifs represented uh more importantly what kind of questions uh do transcription Factor binding site analysis help to answer and lastly I would also want to perform a demonstration using one of the tool that is freely available online I would want to perform motive Discovery and also answer some specific questions um with re

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What are motifs in regulation of gene expression?

Motifs are short (6-8 bases long), recurring patterns that have well-defined biological functions. Motifs include DNA patterns in enhancer regions or promoter motifs, as well as motifs in RNA sequences such as splicing signals. As we have discussed, genetic activity is regulated in response to environmental variations.

What are regulatory motifs in DNA sequence?

Regulatory motifs are short nucleotide sequences typically upstream of genes that are used to control the expression of genes, dictating under which conditions a gene will be turned on or off. Direct identification of regulatory elements is more challenging than that of genes.

What are the motifs that can bind DNA?

Although each of these proteins has unique features, most bind to DNA as homodimers or heterodimers and recognize DNA through one of a small number of structural motifs. The common motifs include the helix-turn-helix, the homeodomain, the leucine zipper, the helix-loop-helix, and zinc fingers of several types.

What are the motifs in DNA recognition?

These motifs include helix-turn-helix (HTH), helix-loop-helix (HLH), zinc fingers, and leucine zippers. HTH and HLH are similar in that they both contain two -helices separated by a turn (short) and a loop (long) region. Both of these usually bind as dimers to inverted repeats in the DNA sequence.

What are motifs in DNA?

A DNA motif refers to a short similar repeated pattern of nucleotides that has biological meaning. Sequence motifs also called regulatory elements exist in Regulatory Region (RR) in eukaryotic gene 2.

What are motifs in biological sequences?

A biological motif, broadly speaking, is a pattern found occurring in a set of biological sequences, such as in DNA or protein sequences. A motif could be an exact sequence, such as TGACGTCA , or it could be a degenerate consensus sequence, allowing for ambiguous characters, such as R for A or G .

What are the binding motifs in proteins?

A binding motif pair consists of two motifs each derived from one side of the binding protein sequences. The discovery is a directed approach that uses a combination of two data sources: 3-D structures of protein complexes and sequences of interacting proteins.

What are binding motifs for transcription factors?

Transcription factor binding motifs (TFBMs) are genomic sequences that specifically bind to transcription factors. The consensus sequence of a TFBM is variable, and there are a number of possible bases at certain positions in the motif, whereas other positions have a fixed base.

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