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hi this is a video tutorial from by informatics review and today we are going to show you how to perform site-specific talking we already have an article published on this but on the special requests from our readers we are going to show you how to use Auto doc tools so for first of all we need to know what are the binding and catalytic sites in a receptor for site specific talking in some cases where these binding sites are unknown we go for blind docking which I will tell you a little later so letamp;#39;s get started we will be following the same tutorial and our protein will be human serum albumin like in will be sodium octan weight and as we can see it is the structure is already complexed with a lichen known as CM PF so first we are going to remove this ligand and then we will talk our ligand in it I will tell you how to do this so we need PDB files for our protein and for our likened the protein PDB file can be downloaded from protein data bank just click on download fi