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in this uh video tutorial I want to discuss with you how we can find ligand binding parameters when we have specific and unspecific binding so in the previous tutorial we have looked at the specific binding aspect of a ligand so letamp;#39;s say weamp;#39;ve got a radio labeled uh ligand and this ligand binds to a receptor it is a reversible reaction and we get a ligand receptor complex and we said we can define a KD for that that is just simply the concentration of the ligand receptor complex divided by the product of the ligand and the receptor we said we can actually model this Behavior and this would be with an absorbance curve so here we would plot the ligand concentration and here we would plot how much is bound so that would be the ligand receptor concentration here that we have and we would usually find a hyperbolic curve where this point here denotes the B max value we also said that half of DP max value so half of B-Max that would give us the ligand concentration he