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all drugs bind to multiple sites on body tissues we aim for the drug to interact with a specific receptor for which it has a high affinity as the result of the drugs ability to interact with a selective binding pocket in the receptor when the drugs come into the proximity of tissues that express their receptors they will bind to the receptors in addition to these receptors drugs also bind to non-specific binding sites on tissues and in the blood the definition of a non-specific binding site is a site to which the drug binds with low affinity and which leads to no effect these non-specific sites can be components of tissue such as proteins that circulate in the blood proteins other than the receptor on the cell surfaces or inside the cells are fat stored in various sites in the body the number of these non-specific binding sites is much greater than the number of receptors the drug binding assay is used extensively in research and in drug development to determine where drugs bind the