Adjust stain in MCW

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How to adjust stain in MCW

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hello everyone welcome to pharma rocks YouTube channel in this video we will solve a multiple-choice questions on subject microbiology usually regarding the bacterial bacterial staining and regarding the microscopy so letamp;#39;s start in this so hello dear friends welcome to pharma rocks youtube channel am arrival here from Parma rocks owner and founder of pharma rocks assistant professor and PhD scholar today we solve most useful mcq from microbiology usually a skin in Japan iPad ein parm assist exam so here is the question number one for you how many magnifying lenses does a compound light microscope content options are option a 1 option B 3 Orson seat to an option D for so right answer for this question is option C 2 so total 2 magnifying lenses does a compound light microscope contains your compound light microscope with a hair you scan the total though magnifying lens is Jo a with a for the magnification purpose question number 2 the presence of a capsule around a bacterial cel

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What factors can modify the expected results of the staining procedure?

Several factors may affect the results of Gram staining: If the smear is too thick, proper decolorizing will not be possible. If the smear is overheated during heat fixing, the cell walls will rupture. Concentration and freshness of reagents may affect the quality of the stain.

What is the correct order of staining reagents in Gram staining Mcq?

Option b) The correct order of staining reagents in Gram-Staining is Crystal violet, iodine solution, alcohol, safranin. Crystal violet is the primary stain that is used to stain the bacterial smear on the slide. Iodine, which is a mordant, supports the adherence of the violet color to the cell wall.

What is the stain of choice for differentiation of bacteria?

Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by colouring these cells red or violet.

What if you forgot to stain with crystal violet?

Answer and Explanation: Normally, Gram-positive organisms appear purple using the Gram stain. However, if you forgot to use crystal violet, they would take up the counterstain (safranin) and appear pink.

What is the importance of crystal violet in Gram staining?

The gram stain utilizes crystal violet as the primary stain. This basic dye is positively charged and, therefore, adheres to the cell membranes of both gram negative and positive cells. After applying crystal violet and waiting 60 seconds the excess stain is rinsed off with water. Next, a mordant is used.

How would that change the outcome of the stain if you were to forget to add crystal violet?

Without the crystal violet staining, it would be impossible to differentiate between Gram-positive and Gram-negative bacteria, as the secondary counterstain, usually safranin, would stain all cells pink instead of just the decolorized Gram-negative cells.

What would happen if you were performing a Gram stain and you forgot to add safranin at the end?

A safranin counterstain is used to stain these Gram-negative cells pink. However, if the safranin counterstain were forgotten, the Gram-negative bacteria would remain unstained, as the original crystal violet stain would have been removed during the ethanol wash, and no additional stain would have been applied.

What would happen if there was a reversal of crystal violet and safranin stains?

If there is a reversal of crystal violet and safranin stains, then the cross-link between iodine and crystal violet wont happen, and thus during decolorization the safranin will fade away, leaving all of the cells colorless.

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Adjust stain in MCW with our multi-function editing tool

How to adjust stain in MCW

PDF editing simplified with DocHub

Drive efficiency with the DocHub add-on for Google Workspace

How to adjust stain in MCW

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Got questions?

What factors can modify the expected results of the staining procedure?

What is the correct order of staining reagents in Gram staining Mcq?

What is the stain of choice for differentiation of bacteria?

What if you forgot to stain with crystal violet?

What is the importance of crystal violet in Gram staining?

How would that change the outcome of the stain if you were to forget to add crystal violet?

What would happen if you were performing a Gram stain and you forgot to add safranin at the end?

What would happen if there was a reversal of crystal violet and safranin stains?

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