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all right now having done a preliminary roi analysis of the optical data in your images you may want to go one step further and determine whether or not there are low level signals in your image that simply arenamp;#39;t being captured because of the default color range threshold being used to set the dynamic range of the signal intensity presented if you look over to the left here the automated color range threshold is well above zero and one can go ahead and do an exhaustive search for data by simply adjusting the color range threshold to zero and the color range minimum of your calibration bar to zero so letamp;#39;s go ahead and double click on the top left image we can adjust the color range minimum to zero and the color range threshold to zero and now we have a fuller presentation of the data that is actually in your image file you can see all of the low-level background signal throughout the image and the big reveal here is that there are above background signals from
