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foreign this tutorial is about how to add a scale bar to an image using image J software here is a confocal image with three different channels green red and blue currently there is no scale bar in this image let us use the image.j software to add a scale bar let us launch the image J software and open the image of your interest to set the scale bar click on analyze tools and scale bar the scale bar dialog box presents a list of parameters let us discuss one after the another at this point there is a scale bar at the lower right of the image the first parameter discuss about the width in pixels by default the width in pixels is set to 160. this parameter can be changed ing to your interest for this tutorial let us put 300 increase in the width of the pixels leads to increase in the length of the scale bar at the lower right the next parameter is height in pixels let us change to 200 we will check the change in the height in pixels in the later part of the video the third parameter is t
