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This ELISA tutorial series is kindly sponsored by Calbiotech. Please click the link below, or in the video description to see what ELISA kits they have to offer. Hi again and welcome to the continuation of our video series on how to perform a sandwich ELISA. So at this point, weve finished coating and blocking the ELISA plate, and now its time to add samples to the plate in order to measure how much of a certain protein there is in those samples. Adding samples to the ELISA plate is probably the most difficult part of performing an ELISA, because it can be overwhelming to deal with a large number of samples. To make this process a little easier, I made a 96-well plate sheet which can be downloaded from the Protocol Place website under the Print-outs tab. This sheet makes it easy to plan where samples must go on the 96-well plate. Organizing samples and standards I like to place my standard curves on the far left and far right sides of the plate, and my samples in the middle region