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this is a video showing the use of the pad pen it is similar to a felt-tip marker only this draws a hydrophobic layer of material around your sample which concentrates the fluid overtop of your sample when we work with aqueous solutions even though water has a good surface tension it still tends to spread out and this dilutes the concentration of any expensive antibody or stain that you may be working with so what were going to do is use a pad pen this pen pen contains 13 milliliters of a hydrophobic liquid that when drawn on the slide forms a barrier to hold aqueous solutions in a single spot when we first get it we need to prime the pen and well notice that by when the felt tip turns nice and red at this point we can draw around our sample at circle in this case or if we have a high aspect ratio sample we can draw it an oval we can draw a square you can draw whatever you want whatever your sample size shape might be so Ive drawn to test circles here o
