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in a previous video we explained that nearly all NGS library prep protocols involve chopping your DNA into small segments called inserts and then attaching little DNA tags to the ends these DNA tags are called adapters and they play a crucial role in NGS and although they sound complex in practice an adapter is simply a small piece of DNA normally around 80 bases in length that is carefully designed with three functional pieces the sequencer binding site and index and a sequencing primer binding site lets look at them each in turn the first segment allows your DNA to bind to the surface of the chip or beads in your sequencer is complementary to a set of allah goes that are already bound to the sequencing chip when it arrives often these sequences are termed P 5 and P 7 P 5 on the five prime end of your insert from P 7 on the three prime 10 difference end next is your index you can sequence DNA from hundreds of samples at a time by NGS but to prevent them getting muddled up each adap
