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okay so weamp;#39;ve set the zoom weamp;#39;ve set the width uh what do we need to worry about next so what we need to worry about next is perhaps one of the most critical parameters in light sheet imaging with this system which is the sheet n a so to explain how this sheet in a business works iamp;#39;m going to engage a few things here first iamp;#39;m going to click on horizontal focus if i click there youamp;#39;ll see that now there is this sort of bow tie shape at the top of my imaging window so what that bow tie shape represents is the shape of the light sheet in the z dimension so if i go back here to the guide the light sheet is not actually flat instead if you look at it uh sort of along x z it has a waste in z and then it flares out so in the system remember the sample is in here so itamp;#39;s sort of sitting there and the light sheet is coming like this but itamp;#39;s actually not flat it has kind of a bow tie shape so it starts out thick then it gets thin and then
