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hi in this video I will teach you how to use image a in order to process can focal microscopy data so you have our data of confocal microscope a confocal microscope image the conferral microscope would give you a composite image of all the channels and now for representing it in a paper you have to do a lot of processing you have to split the channels so lets open one data so here we will open one data one image of confocal a composite image so here you can see after clicking on the image and open image would open so this is the conferral image RGB image you can see it saves RGB and it also gives the pixel value of this particular image and now what we would do would split the channels and see what what is in green and what is in red and then we would give a March and we would place three side-by-side for better understanding so in order to perform this thing first we have to get to image and then colors there we have to change the option to make composite so when we make it composite