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in this video I will explain the function of binary expression systems in Drosophila binary expression systems allow scientists to investigate the complex spatiotemporal expression pattern of genes of interest they also allow scientists to investigate the role of specific proteins by allowing for the increase or knockdown of protein expression in specific cells because this is a binary expression system we know that it will consist of two independent pieces which are a trans activator and a reporter so we start with two lines of flies the first line called the driver line contains a p element insertion that encodes a trans activator a protein that serves to activate expression of another gene this trans activator will be under the influence of a native promoter or enhancer and it will only be expressed in cells of the organism in which the enhancer is active for example if the trans activator is under the control of a promoter that is only active in insulin producing cells which we cal