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calibrating the zero pressure offset on your ekta avant or ekta pure is a vital part of maintaining your instrument to ensure it is operating at peak performance an accurate zero pressure offset is necessary for precise and consistent pressure readings it is good practice to calibrate the pressure offset weekly there are four pressure sensors on the ecta avant and ectapur may have anywhere between one and four pressure sensors depending on the configuration in unicorn navigate to the system control window and click on the system tab at the top of the page select calibrate from the drop down menu in the calibration window select the pressure sensor you wish to calibrate from the drop down menu an information window in the lower right corner provides detailed instructions for performing the calibration remove the fitting and tubing on the pressure sensor you wish to calibrate to release any internal pressure for the system or sample pump remove the tubing on top of the pressure s
