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hello good afternoon everyone welcome to liquid brain so today i want to talk about the first step in every single cell rna-seq project which is after you get back your data how to identify how many cluster is in your sample in this case how many cell type are they what kind of marker can you use to differentiate them into different kind of cell as well as some like some basic you know quality control and normalization and qc stuff so this is what were looking for in the end so this is a umap plot of all the nine different clusters as well as the cell type so if you do not like unap we also will be doing something like a pca well be doing something like heat map and some you know the the cell expression in different cluster and so on and so forth so well be using some a package called this silhouette sailrite something some of that that some sort like that so its actually developed by satya lab so you can actually get the original tutorial and guideline in the website above so the