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This video tutorial explains how to conduct a t-test in Excel to compare differences between two conditions based on reaction times measured in milliseconds. The tutorial outlines two scenarios: both conditions can involve the same participants (e.g., measuring morning vs. afternoon reaction times) or different participants for each condition. If the means come from the same subjects, a related t-test (paired t-test) is used. If separate subjects are tested in each condition, a different type of t-test is applicable. The video will guide viewers through using Excel to perform the t-test based on these considerations.