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This video tutorial demonstrates how to remove an intraosseous (IO) line. Key learning objectives include identifying the necessary equipment and demonstrating the proper removal procedure. Indications for removal of the IO line are: the patient no longer requires it, the line is malfunctioning, or it has been in place for more than 24 hours. Typically, IO access should be used for less than 24 hours or until long-term access is available. Required equipment includes a procedure mask, protective eyewear, gloves, sterile transparent dressing, a 10-milliliter sterile syringe, a sharps container, and tape. The removal process begins with hand hygiene and donning PPE, followed by detaching the extension tubing and placing it in a sharps container, then using the sterile syringe as a handle for removal.