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[ Music ] Today we are talking about column chromatography. Everything I do, should be done in a hood because you are using volatile organic solvents. So, for video purposes we are doing this on the benchtop but remember always work in a hood. You are already familiar with a TLC which is a separation technique that uses silica gel chromatography. TLC is an analytical technique meaning all you get out of this technique is information. We are using way to little compound for it to be practically useful. If you want to have a sizeable amount of compound you have to scale it up. So, to scale it up, you dont use just a very thin layer of silica on a plastic backing. You use a lot of silica and you pack a column of it. Column chromatography is a very touchy technique. There are three phases in column chromatography. First, you pack the column. That is, you prepare it for you experiment. Then, you load it. Which is the equivalent of spotting the TLC plate that is you put your compound

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