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Weve examined a handful of biotechnology concepts in previous tutorials, but now its time to introduce what is undoubtedly the most promising technique in biotechnology of the past decade. The CRISPR-Cas9 system represents genome editing technology that has revolutionized molecular biology, due to its precise and site-specific gene editing capabilities, which essentially allow for an unprecedented level of control in manipulating the genetic information of a living organism. How does this work mechanistically, and what are its applications? Lets get a closer look now, starting with some historical context. In 1987, Atsuo Nakata and his team of researchers from the Osaka University in Japan first reported the presence of Clustered Regularly Interspaced Short Palindromic Repeats, abbreviated as CRISPR, in the Escherichia coli genome. These refer to short, repeated sequences of DNA nucleotides found within the genome of prokaryotes. These sequences are the
