Insert stain in PAP

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Add your PAP file into your DocHub account.
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Once finished, click Download/Export and save your PAP to your device or cloud storage.
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How to insert stain in PAP

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hey whatamp;#39;s up everybody welcome back to my youtube channel todayamp;#39;s video weamp;#39;re going to talk about colposcopies so just a little bit of a background in case you havenamp;#39;t seen my previous video where i talk about pap smears exactly how we do them and i explain all of the different results that you might see with a pap smear this oneamp;#39;s gonna be a part two to this video because if you have an abnormal pap smear you may end up needing a procedure called a colposcopy so in todayamp;#39;s video iamp;#39;m gonna be explaining the whole process of a colposcopy how we do them and iamp;#39;m super excited about this video because iamp;#39;m also going to share a little behind the scenes of what it actually looks like to get a colposcopy iamp;#39;m going to show you exactly the procedure room that i use to do colposcopies in my office iamp;#39;ll get to show you the culpable scope the instruments we use the whole process so that in the event that you d

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What is inserted during a Pap smear?

During a Pap test, a tool called a speculum holds the vaginal walls apart. A sample of cells from the cervix is collected using a soft brush and a flat scraping device called a spatula (1 and 2). The cells are placed in a bottle that contains a solution to preserve them (3).

What is the difference between a Pap smear and a Pap stain?

Typically, we first think of Pap tests (Pap smears) as a specific type of cytological test identifying cancerous cells in cervical tissue. However, Pap stains can also be conducted on non-gynecological specimens from a variety of bodily secretions and from small needle biopsies of organs and tissues.

What is used for blueing in Pap stain?

PAP bluing solution is a variant of Scotts solution and is used specifically in Papanicolaou staining (Pap staining) for cytological smears. It is used to bluish the hematoxylin in the stained nuclei to improve contrast and visibility, which facilitates the interpretation and analysis of cytology specimens.

What is the stain for a Pap smear?

The Papanicolaou stain is undoubtedly the most commonly used in cytology. It has three cytoplasmic dyes, orange G, eosin Y, and light blue. It can stain cells blue or blue-green (basophilia, cyanophilia), pink (acidophilia, eosinophilia), orange (orangeophilia), or indeterminate (gray-blue).

How to destain a Pap stain?

Wash slides in deionized or distilled water for 5 min at room temperature. Destain slides in 0.5% HCl in 70% ethanol for 5-15 min at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature. Air dry sections.

What are the solutions to the Pap stain?

The conventional Pap stain has 24 solutions, of which three are acid dyes, six water, one is chemically defined bluing agent, eight 95% ethanol, three absolute alcohol and three xylene Gill, 2006. The cytomorphology with conventional Pap stain is shown in Figure 2 .

How do you remove pap stains?

Procedure: Remove cover slip by incubation in xylene for a few hours to several days (until the coverslip can be removed easily). Rehydrate slides in 100%, 85%, and 75% ethanol, each for 30 sec at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature.

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