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the overall goal of this methodology is to assess the susceptibility of influenza a and b viruses to the neuraminidase inhibitors by using a fluorescence-based assay this method can help determine whether an influenza virus is resistant or sensitive to the neuramininase inhibitor class of antivirals the main principle of the assay is to see whether the neuraminilase inhibitor antiviral blocks the action of the neuraminidase enzyme activity prior to antiviral susceptibility testing the virus must be must be grown to high viral tita by either passaging in cell culture such as the mdck cells or in embryonated chicken eggs in this demonstration i will be using live influenza a viruses of the h1n1 and h3n2 subtypes beginning with a 96-well u-bottom plate dispense 120 microliters per well of undiluted cultured influenza viruses into column one and 60 microliters of one times assay buffer containing 0.1 percent np40 into the remaining 11 columns using a multi-channel pipette serially
