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unopened insulin can be stored in the refrigerator and will expire by the date on the box write the date on the bottle on the day you start using it open bottles of insulin can be kept at room temperature most insulin vials are good for 28 days but always check with your pharmacist to find out how long your insulin vial is good for wash your hands if the insulin bottle is cloudy roll the bottle from side to side for one minute do not shake the bottle if your insulin is clear you do not need to roll the bottle remove the plastic cap if youamp;#39;re opening a new bottle of insulin if the bottle is open use an alcohol wipe to clean the top of the bottle remove the tops from both the top and bottom of the syringe do not touch the needle pull the plunger on the syringe back to the amount of your insulin dose put the syringe straight into the bottle and push the plunger down turn the bottle upside down with the syringe still inside while holding the bottle at eye level pull back on the plu