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for the hlc you use different solvents to dilute your sample Iamp;#39;ve labeled these as 10 star 20 Star 50 star the number indicates the percent aceto nitri in these mixtures the star indicates they also have Trio acetic acid itamp;#39;s an acidulant itamp;#39;s 0.1% for the Esters the recommended d went will be 50 star 50% water 50% aceto n trial and Iamp;#39;ll make the dilution in an endorf tube I have here a stock solution of1 M salicylic acid to make the delution uh Iamp;#39;ll do a 50 fold dilution which will make the concentration in the endorf tube 2 molar Iamp;#39;m using a micro capillary that actually measures well from a box like this it comes with a plunger when itamp;#39;s assembled it looks like this and I can pull the plunger up and there are lines here that Mark the microliters so Iamp;#39;ll get four microliters and Iamp;#39;ll dilute it to 200 thatamp;#39;s a 50 fold dilution in this case itamp;#39;s not critical I get a precise amount um I could use a P
