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so once you have your syringe prepared the samples in it and the software is loaded and you have everything set up to go with the software you can inject the sample just hold the syringe vertically this needle goes into a little silver piece which is on top of a green metal cap here inside of that thereamp;#39;s a rubber septum so when you push the needle through thereamp;#39;s this rubber septum you have to push through so just gently push it through inject it and pull it out quickly okay so once again needles ready to go syringe is ready to go inject it out you want to do that within a few seconds you