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glass capillary pipettes have become ubiquitous in laboratories for a wide variety of techniques such as single cell electroporation dna micro injection and patch clamp electrophysiology to prepare glass capillary patch clamp electrodes for recordings four silicate glass is pulled on a programmable puller the electro tips are coated with wax then the tips are fire polished and stored for future use hi iamp;#39;m miriam goodman from the department of molecular and cellular physiology at stanford university in this video i will demonstrate how to make patch clamp pipettes and sharp electrodes for electrophysiology glass microelectrodes also called pipettes have been a workhorse of electrophysiology for decades today these pipettes are made from glass capillaries using a programmable puller so letamp;#39;s get started to make patch clamp pipettes we use sutter bora silica capillaries that are clean and dust free and we use the sutter p97 flaming brown micro electrode puller solderamp;#
