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transfer the frozen tissue block to a cryo stat at minus 20 degrees Celsius and allow the temperature of the frozen tissue block to equilibrate to the temperature of the cryo staff apply Oct compound to fix the tissue block to the holder fix the tissue block to the arm and adjust the settings to the desired angle and thickness you attach the tissue sample to a slide approved for immunostaining dry the slides at room temperature for 1 hour before staining or store at minus 20 degrees Celsius for future use use a PAP pen to draw a hydrophobic barrier surrounding the tissue to keep the staining reagents and buffers within the selected area fix tissue sections with 4% para formaldehyde for ten minutes at room temperature then wash the slides in PBS three times five minutes each for chroma genic staining xamp;#39; block endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide solution in methanol at room tem