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hello my name is jun today we like to go over some tips and tricks in how to use our melee cell cm inserts these have a ptfe membrane so there are certain tips and tricks theyamp;#39;ll be very helpful for you in terms of preparing these inserts for your asset this is how the insert is packaged and what it looks like the insert contains a hydrophilic ptfe membrane this membrane in general is less hydrophilic compared to other commonly used pet or pc membrane inserts due to its reduced hydrophilicity it is recommended to place the insert in certain orientation first letamp;#39;s prepare a six well plate containing about one milliliter of media it is recommended to use warm media as cold media can hinder the waiting processes now the media does not need to be exactly at 77 degrees celsius but it is warm to touch now letamp;#39;s place the insert into the well as you can see here it is best to place the insert slightly at an angle as to avoid trapping of bubbles there you go now you ha
