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hello and welcome to the shiki-san show in this video well look at prime editing why the hype so Im not entirely sure why I havent done a video on gene editing and CRISPR calf sign systems already since I like to think of myself a little bit of a Chris Forex birds Im joking its not really quite the case but Im I have worked with and read a lot of literature to deal with CRISPR Castine so I was intrigued when a paper came out last week which was all about this new concept called prime editing but before I jump stratum of prime meditating lets first take a look at Chris forecast 9 and some of its limitations that kind of kick started this publication so Chris Picasa 9 in the context of genome editing involves a protein called Castine that has endonuclease activity such that it can cut DNA and this can induce a double-stranded break into the DNA and then thats double trying to break these be repairs and that is why editing can occur in correct conditions and to get to that target