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hi my name is Ray if you are interested in learning how to use parenchymal airspace profiling method and the software then you are in the right place i'm going to show you my usual workflow hope this video can help you with your future research ok now after you collect a 10x equivalent image that represent your slides you can open this this follow-up in adobe photoshop here you can edit this file before you feed them into the parenchymal aerospace profiling software generally i do two types of things first to clean a relevant stuff out of the image and also to paint the non pragma area with a particular color so later in the software it will automatically recognize the color and count them towards non pragma area ok to do the cleanup let's just press B to switch the paint brush ok if you do not have the foreground color selected correctly you can hold down alt and sample an area as soon as you click this foreground color is going to turn exactly into that into that color and then just...