Cut stain in MCW

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Aug 6th, 2022
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How to cut stain in MCW

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dear learners today we are going to talk about the mcqs from microbiology and the topic is bacteria we are going to cover part 3 few of the mcqs from the bacteria in our earlier video part one and part two we have seen few possible mcqs on the same topic that is bacteria so to start with part three first question is the question says cell wall of gram negative bacteria is whether it is thick gram negative they are asking whether it is thick whether lipids are present whether ticoic acid is absent or none of them thick it is correct lipids present is correct acid are absent and none of these the correct answer is it is ticoic acid is absent now why as you can see the structure see this is gram positive and this is gram negative in gram positive you can find lipotic acid acid so this is cytoplasmic membrane above that peptidoglycan is layer in gram negative there is very less peptidoglycan and lipopolysaccharide is there lipoprotein and above that there is o antigen core lipidae which is

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Similar to Types of staining techniques in microbiology It then describes the basic components of stains and dyes. The document outlines different types of staining including simple, differential, Gram, acid-fast, capsule, and endospore staining.
Intermolecular attractions are what causes a stain to adhere to bacterial cells. Stains which are polar in nature will interact with polar molecules in the bacterial cell wall while nonpolar stains will interact with nonpolar molecules.
Stains Smear Preparation. Simple Stain. Negative Stain. Gram Stain. Congo Red Capsule Stain. Wirtzs Endospore Stain.
Commonly used negative stains include uranyl acetate (UA), uranyl formate, ammonium molybdate, and phosphotungstic acid. The technique was invented by Brenner and Horne (1959) and first applied on mosaic viruses mixed with phosphotungstic acid.
Special Stains Massons Trichrome. The trichrome stain helps to highlight the supporting collagenous stroma in sections from a variety of organs. Verhoffs Elastic Stain. Reticulin Stain. Giemsa Stain.
What is the correct order of staining reagents in Gram-Staining? Explanation: Gram staining is a type of differential staining. In this process the fixed bacterial smear is subjected to the following staining reagents in the order listed: crystal violet, iodine solution, alcohol (decolorizing agent), and safranin.
The final step in gram staining is to use a basic fuchsin stain to give decolorized gram-negative bacteria a pink color for easier identification. It is also known as counterstain. Some laboratories use safranin as a counterstain; however, basic fuchsin stains gram-negative organisms more intensely than safranin.
Reagents needed for Gram staining include: Crystal violet (primary stain) [1] Grams iodine solution (the mordant) [1] Acetone/ethanol (50:50 v:v) (the decolorizer) [1] 0.1% basic fuchsin solution (the counterstain) [1] Water.

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