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Monarch RNA Cleanup Kits can be used to purify RNA from in vitro transcription reactions or other enzymatic reactions, such as DNase I treatment. We offer 3 kits for RNA cleanup, each with a different capacity: 10 g, 50 g, and 500 g. This video will provide tips to optimize performance from all 3 kits. Be sure to add the RNA Cleanup Binding Buffer mix well, and then add ethanol before loading the sample onto the column. The binding buffer serves to inactivate enzymatic activities, thereby preserving sample integrity. So, it is important to add this buffer first. The addition of ethanol ensures that the RNA binds to the column. Do not load 900 l on the column at one time, as this will exceed the column reservoir capacity. To optimize the quality of your purified RNA, ensure that the tip of the column does not come in contact with the collection tube after washing- doing so may introduce buffer components into your RNA. When eluting, add nuclease-free water directly to the center o
