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once the proteins have been separated in the gel they can now be transferred to a solid support membrane to start the Western blotting workflow in this video youamp;#39;ll learn how to perform a Western transfer using the mini blot module in a mini gel tank make sure that cassette clamps are removed before you set up your transfers one gel is transferred per module into modules fit in one tank included with the mini blot module kit is a roller sponge pads tweezers in a quick reference card approximately 250 milliliters of transfer buffer is required for each transfer prepare transfer buffer by adding 25 milliliters of 20 X bolt transfer buffer 50 milliliters of methanol 500 microliters of bolt antioxidant and bring the total volume up to 500 milliliters with deionized water soap to sponge pads and transfer buffer and squeeze the pads to remove air bubbles open the gel cassette using a gel knife and carefully remove the wells and the foot of the gel so that the entire gel is of
