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here are results of the protein gel this is where you start your analysis this is the marker lane so this is Lane two three four five six seven eight I this is my record of lane assignments two and three are sharp four is Liberty five as markers six is Ravina seven Campos 8 is Hoffs interpretation of the markers is here the smallest band is ten thousand grams per mole ten kilo daltons 15 20 25 20 and 25 they got to get to 37 then 50 75 100 150 250 so you use this as your standard for figuring out what protein molecular weights do you have in your sample so Ill use this one its fairly clear this band here is between the 50 and the 75 killer Daltons so its probably something like 55 KD 55,000 is its molecular weight these two bands on the other hand are between 37 and 50 so maybe this is 39 and 41 somewhere in that range their molecular weight the beta lactamase itself the enzyme were looking for is 27 killa Daltons so I go to the 25 and the 37 heres the 25 is the 37 Im thinking