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today iamp;#39;m going to be speaking to you about how alignment is performed within our 2d analysis software same spots the first step in aligning your 2d gel and blot images within same spots is choosing a reference image so the reference image can either be selected manually by using these buttons down here on the side next to the name of the jello block image that youamp;#39;ve imported into the project file or it can be selected automatically by default by the software so as you can see um the same spot software has analyzed all of the images that iamp;#39;ve brought into the project and itamp;#39;s selected based on the intensity and the clarity of the spots within the image which one would make the best reference image to align all of my other images too so you can see in this instance itamp;#39;s automatically selected uh filename gel4 internal standard cy2 stain however if i did want to override that it would be as simple as clicking one of these other images to use as yo
