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A dot blot is a method for quickly detecting biological samples such as proteins or nucleic acids. It is similar to Western blotting and Southern blotting, but the sample is dotted directly onto a membrane before detection. This simple method is used for various applications, including monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency, a sample labeled with biotin is compared to a known biotinylated reference standard through serial dilutions of both samples.